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RNase Inhibitor, GMP Grade(Cat. No.:GMP-E125)
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Description
  • RNase Inhibitor inhibits RNase A, B and C with high efficiency by forming a 1:1 complex with them. The characteristics of RNase Inhibitor are similar to those derived from pig liver and human placenta. The inhibition is reversible, as the complex can be dissociated by urea and sulfhydryl reagents in which RNase refolds and the inhibitor is inactivated. It can be directly added to the reaction containing RNA. Different from other competitive inhibitors (e.g., nucleic acids, inorganic phosphoric acids), this product is a protein, which can be easily removed from the reaction by phenol extraction. However, the reverse transcriptase RNase H could not be inhibited.
  • Recombinant RNase Inhibitor is GMP Grade produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.
  • This product has completed the DMF record of FDA and passed the HALAL certification.
Usage
  • 1. Synthesis of cDNA 
  • 2. In-vitro translation 
  • 3. Separation of polyribosomes
  • 4. In-vitro transcription
Quality Control
ElementStandard
AppearanceClear and transparent solution
IdentificationPositive
Visible ParticlesMeet the specification
pH7.0-8.0
Activity39KU/ml-41KU/ml
Purity≥95%
Protein ContentMeet the specification
Endonuclease ResiduesThe degradation of substrate was ≤10%
Exonuclease ResiduesThe degradation of substrate was ≤10%
RNase ResiduesThe degradation of substrate was ≤10%
Bacterial Endotoxins<5EU/ml
Exogenous DNA Residues≤100pg/mg
Host-cell Protein Residues≤50ppm
MycoplasmaNegative
Heavy Metals≤10ppm
Microbial LimitTotal aerobic microbial count≤1cfu/10ml, total yeasts and molds count≤1cfu/10ml
Storage
At -20±5℃.
Components
SKUComponentsVolume
GMP-E125-01ARNase Inhibitor, GMP Grade (40U/μl)75μl
GMP-E125-M001RNase Inhibitor, GMP Grade (40U/μl)1ml
GMP-E125-M010RNase Inhibitor, GMP Grade (40U/μl)10ml
GMP-E125-M050RNase Inhibitor, GMP Grade (40U/μl)50ml
Notes
RNase inhibitor has a wide range of pH values (activity was found between pH5.0-9.0), with maximum activity at pH7.0-8.0, isoelectric point at 4.7.
Related Products
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GMP-M062Vaccinia Capping System, GMP GradeGMP-E121T7 RNA Polymerase, GMP Grade
GMP-M072mRNA Cap 2´-O-Methyltransferase, GMP GradeGMP-E127DNase I, GMP Grade
GMP-M012E. coli Poly(A) Polymerase, GMP GradeGMP-M036Pyrophosphatase, Inorganic (yeast), GMP Grade
GMP-E131T7 RNA Transcription Enzyme Mix, GMP GradeGMP-S023-026NTPs, GMP Grade
仅供科研或生产使用,不可直接应用于人体。
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