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10×Capping Reaction Buffer, GMP Grade(Cat. No.:GMP-EB62)
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Description
10×Capping Reaction Buffer, GMP Grade is the companion reaction buffer for the Vaccinia Capping Enzyme, GMP Grade (Cat. No.: GMP-M062) and mRNA Cap 2´-O-Methyltransferase, GMP Grade (Cat. No.: GMP-M072). The formula of 1×Capping Reaction Buffer is 50mM Tris-HCl (pH 8.0 at 25℃), 5mM KCl, 1mM DTT, 1mM MgCl2.
Features
  • Versatility: Suitable for enzymatic capping reactions for Cap0 and Cap1 RNA synthesis.
  • Efficiency: This system can be used for the capping reaction of RNA produced by T7 RNA Polymerase (GMP-E121, Novoprotein), so that the capping reaction is completed within one hour, the efficiency is close to 100%, and the correct direction is guaranteed.
Quality Control
ElementStandard
AppearanceClear and transparent solution
Visible ParticlesMeet the specification
pH7.5-8.5
Endonuclease ResiduesThe degradation of substrate was ≤10%
Exonuclease ResiduesThe degradation of substrate was ≤10%
RNase ResiduesThe degradation of substrate was ≤10%
Non-Specific DNase ResiduesThe degradation of substrate was ≤10%
Bacterial Endotoxins<1EU/ml
MycoplasmaNegative
Heavy Metals≤10ppm
Microbial LimitTotal aerobic microbial count≤1cfu/10ml, total yeasts and molds count≤1cfu/10ml
Storage
At -20±5℃.
Components
SKUComponentsVolume
GMP-EB62-01A10×Capping Reaction Buffer, GMP Grade1ml
GMP-EB62-M00510×Capping Reaction Buffer, GMP Grade5ml
GMP-EB62-M01010×Capping Reaction Buffer, GMP Grade10ml
GMP-EB62-M05010×Capping Reaction Buffer, GMP Grade50ml
Notes
  • Please mix upside down gently (avoid foaming) and slight centrifugation before use. For the preparation and dispensing of the reaction solution, please use a new (RNase-free) pipette tip, microtube, etc., to ensure that all reagents are free of RNase pollution.
  • To ensure the efficient reaction, high-quality DNA template should be used and operated on ice.
  • For your safety and health, please wear a lab coat and disposable gloves.
Related Products
Cat. No.Product NameCat. No.Product Name
GMP-M062Vaccinia Capping Enzyme, GMP GradeGMP-E125RNase Inhibitor, GMP Grade
GMP-M072mRNA Cap 2´-O-Methyltransferase, GMP GradeGMP-E127DNase I, GMP Grade
GMP-M012E. coli Poly(A) Polymerase, GMP GradeGMP-M036Pyrophosphatase, Inorganic (yeast), GMP Grade
GMP-E121T7 RNA Polymerase, GMP GradeGMP-E131T7 RNA Transcription Enzyme Mix, GMP Grade
GMP-S023-026NTPs, GMP Grade
仅供科研或生产使用,不可直接应用于人体。
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