Recombinant Human CD3D&CD3E Heterodimer (C-6His)

Cat.No.: C01S

Recombinant Human CD3D&CD3E Heterodimer (C-6His)
Description
Recombinant Human CD3D&CD3E is produced by our Mammalian expression system and the target gene encoding Phe22-Ala105&Asp23-Asp126 is expressed with a 6His tag at the C-terminus.
Accession #:P04234&P07766
Known as:CD3E & CD3D; CD3 delta & CD3 epsilon; CD3 delta / epsilon
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.
Quality Control
Purity:Greater than 95% as determined by reducing SDS-PAGE.
BioActivity:Immobilized Human Anti-CD3-Fc(Cat#A018) at 10μg/ml (100 μl/well) can bind Human CD3E-His(Cat#C01S).
Endotoxin:Less than 0.1 ng/?g (1 EU/?
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background
T-cell surface glycoprotein CD3D & CD3E, also known as CD3 delta & CD3 epsilon chain, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

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