Recombinant Human Ciliary Neurotrophic Factor/CNTF

Cat.No.: C098

Recombinant Human CNTF
Description
Recombinant Human Ciliary Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding Ala2-Met200 is expressed.
Accession #:P26441
Known as:Ciliary Neurotrophic Factor; CNTF
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, 1mM Cysteine, pH7.4.
Quality Control
Purity:Greater than 95% as determined by reducing SDS-PAGE.
BioActivity:Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
Endotoxin:Less than 0.1 ng/?g (1 EU/?g) as determined by L
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background
Ciliary Neurotrophic Factor (CNTF) is a potent survival factor for neurons and oligodendrocytes. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL6, IL11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal transducing subunit in their receptor complexes. CNTF, like FGF acidic, FGF basic, and PD-ECGF (platelet-derived endothelial cell growth factor), does not possess a signal sequence that would allow secretion of the factor by classical secretion pathways. The mechanism underlying the release of CNTF is unknown.

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