Recombinant Human Fibroblast Growth Factor 7/FGF-7/KGF

Cat.No.: CH73

Recombinant Human KGF
Description
Recombinant Human Fibroblast Growth Factor 7/Keratinocyte growth factor is produced by our E.coli expression system and the target gene encoding Cys32-Thr194 is expressed.
Accession #:P21781
Known as:Fibroblast growth factor 7;FGF-7;Heparin-binding growth factor 7;HBGF-7;Keratinocyte growth factor;FGF7;KGF
Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM Tris HCl,150mM NaCl,1mM EDTA,pH8.0.
Quality Control
Purity:Greater than 95% as determined by reducing SDS-PAGE.
BioActivity:Immobilized Human KGF(Cat#CH73) at 2μg/ml (100 μl/well) can bind Human FGF R3-Fc(Cat#CB43).The ED50 of Human KGF(Cat#CH73) is 0.5-2 ug/ml .
Endotoxi
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background
Fibroblast growth factor 7 (FGF7) is a secreted protein which is mainly located in epithelial cells and belongs to the heparin-binding growth factors family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. It is possible major paracrine effector of normal epithelial cell proliferation.

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