Recombinant Mouse Hepatocyte growth factor/HGF (C-6His)

Cat.No.: CC13

Recombinant Mouse HGF (C-6His)
Description
Recombinant Mouse Hepatocyte growth factor is produced by our Mammalian expression system and the target gene encoding Gln33?Leu728 is expressed with a 6His tag at the C-terminus.
Accession #:Q08048
Known as:Hepatopoietin-A;Scatter factor;SF;deafness, autosomal recessive 39; DFNB39;F-TCF; hepatocyte growth factor
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Quality Control
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background
HGF, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60?kDa alpha ?and 30 kDa beta ?chain. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. ?It can also alter epithelium morphology by the induction of nectin?1 alpha ?ectodomain shedding, an adhesion protein component of adherens junctions?.

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