Recombinant Human Interleukin-2/IL-2/aldesleukin

Cat.No.: C015

Recombinant Human IL-2 (aldesleukin)
Description
Recombinant Human Interleukin-2(C145S) is produced by our E.coli expression system and the target gene encoding Pro22-Thr153(Cys145Ser) is expressed.
Accession #:P60568
Known as:Interleukin-2; IL-2; T-cell growth factor; TCGF; Aldesleukin
Formulation
Lyophilized from a 0.2 μm filtered solution of 10mM Acetata-Na, 5% Trehaiose, pH 4.5.
Quality Control
Purity:Greater than 95% as determined by reducing SDS-PAGE.
BioActivity:Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 90-270 pg/ml
Endotoxin:Less than
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background
Recombinant Human Interleukin-2 is a highly purified protein with a molecular weight of approximately 15,300 Daltons. The chemical name is des-alanyl-1, serine-145 Human Interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the Human IL-2 gene, and the resulting expression clone encodes a modified Human IL-2. This recombinant form differs from native Interleukin-2 in following ways: it is not glycosylated; the molecule has serine substituted for cysteine at amino acid position 145; the aggregation state of molecule is likely to be different from that of native IL-2.

Send Message