Recombinant Human cTn I is produced by E.coli expression system and the target gene encoding Met1-Ser210 is expressed with a 6His tag at the N-terminus.
cardiac troponin-I,Human Cardiac Troponin I
AP Mol Mass:
30kDa, reducing conditions
Greater than 95% as determined by reducing SDS-PAGE.
Supplied as a 0.2 μM filtered solution of 20mM Tris,100mM NaCl,1mM DTT,10% Glycerol,pH 8.0.
The product is shipped on dry ice pack. Upon receipt, store it immediately at the temperature listed below.
Reconstituted protein solution should be stored at ≤ -20°C.
Affinity purification chromatography.
Immunogen, calibrator or standard.
Troponin is a heterotrimer that regulates muscle contraction in skeletal and cardiac muscle (but not in smooth muscle). Troponin acts with intracellular calcium to control the interaction of actin and myosin filaments in striated muscle fibers. Though they perform similar functions, cardiac and skeletal troponins differ in sequence and can be differentiated in immunoassays. When muscle tissue is damaged, the troponin-tropomyosin complex breaks down and troponin I and troponin T are released into the blood. Cardiac troponin T (cTnT) and cardiac troponin I (cTnI) can be readily distinguished from their skeletal muscle analogs allowing confirmation of cardiac muscle tissue damage over skeletal muscle tissue damage. Troponins are excellent biomarkers for myocardial injury in cardiotoxicity because of the demonstrated tissue-specificity of cardiac and skeletal troponins.
FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY