Recombinant HIV p24 protein is produced by E.coli expression system and the target gene encoding Met142-Gly355 is expressed with a 6His tag at the C-terminus.
Capsid protein p24;Human immunodeficiency virus 1;Human immunodeficiency virus type 1 p24
AP Mol Mass:
25kDa, reducing conditions
Greater than 95% as determined by reducing SDS-PAGE.
Supplied as a 0.2 μM filtered solution of PBS, pH7.4.
The product is shipped on dry ice pack.Upon receipt, store it immediately at the temperature listed below.
Reconstituted protein solution should be stored at ≤ -20°C.
Affinity purification chromatography.
Confirmed by reaction with monoclonal antibodies specific to p24.
Immunogen, calibrator or standard.
The HIV1 p24 protein performs highly complex orchestrated tasks during the assembly,budding maturation and infection stages of the viral replication cycle. During viral assembly,the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag p24, the major capsid protein of the HIV-1 virion, has been used in clinical trials as one of the components of the HIV-1 vaccine because of the high degree of sequence conservation between different isolates. Recombinant HIV1-p24 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.
FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY