Recombinant Human Jo-1 is produced by E.coli expression system and the target gene encoding Met1-Cys509 is expressed with a 6His tag at the N-terminus.
Histidyl-TRNA Synthetase;HARS;CMT2W;HRS; USH3B;HisRS;Histidine Translase
AP Mol Mass:
50-60kDa, reducing conditions
Greater than 95% as determined by reducing SDS-PAGE.
Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 10% glycerol, pH8.0.
The product is shipped on dry ice pack. Upon receipt, store it immediately at the temperature listed below.
Reconstituted protein solution should be stored at ≤ -20°C.
Affinity purification chromatography.
Immunogen, calibrator or standard.
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene.
FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY