Recombinant human NT-proBNP is produced by E. coli expression system and the target protein encoding His27-Arg102 is expressed of Human NPPB fused with a 6His tag and FLAG tag at the N-terminus.
Amino terminal human brain natriuretic peptide precursor, NT-proBNP
AP Mol Mass:
14kDa, reducing conditions
Greater than 95% as determined by reducing SDS-PAGE.
Supplied as a 0.2 μM filtered solution of PBS, pH7.4.
The product is shipped on dry ice pack. Upon receipt, store it immediately at the temperature listed below.
Reconstituted protein solution should be stored at ≤ -20°C.
Affinity purification chromatography.
Confirmed by reaction with monoclonal antibodies specific to different fragments of NTproBNP.
Immunogen, calibrator or standard.
Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12 kDa BNP precursor gives rise to N-terminal Pro BNP (NT-proBNP) and mature BNP. N-terminal proB-type natriuretic peptide (NT-proBNP), a useful marker of heart failure (HF), is considered to be secreted mainly from the ventricle, increased serum NT-proBNP levels are also encountered in conditions such as atrial fibrillation (AF) and atrial septal defect in patients without HF.
FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY