Recombinant TB antigen CFP-10 is produced by our E.coli expression system and the target gene encoding Met1-Phe100 is expressed with a 6His tag at the N-terminus.
ESAT-6-like protein EsxB; 10 kDa culture filtrate antigen CFP-10; CFP-10
AP Mol Mass:
14kDa, reducing conditions
Greater than 95% as determined by reducing SDS-PAGE.
Supplied as a 0.2 μM filtered solution of PBS, pH 7.4.
The product is shipped on dry ice pack.Upon receipt, store it immediately at the temperature listed below.
Reconstituted protein solution should be stored at ≤ -20°C.
Affinity purification chromatography.
Immunogen, calibrator or standard.
CFP-10,a secreted protein,acts as a strong host (human) T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA; native protein binds poorly to artificial liposomes in the absence or presence of EsxA. EsxA and EsxA-EsxB are cytotoxic to pneumocytes. EsxB (and EsxA-EsxB but not EsxA alone) activates human neutrophils; EsxB transiently induces host (human) intracellular Ca2+ mobility in a dose-dependent manner, monocytes and lymphocytes do not respond. Neutrophils respond to EsxB by chemotaxis and primed neutrophils treated with EsxB produce reactive oxygen species (ROS); Ca2+ release and the ROS burst via are induced by an unidentified G-protein coupled receptor. May help regulate assembly and function of the type VII secretion system (T7SS).
FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY