Recombinant Human Fc gamma RI/FCGR1A/CD64 (C-6His)

Cat.No.: CM60

Description
Recombinant Human High affinity immunoglobulin gamma Fc receptor I is produced by our Mammalian expression system and the target gene encoding Gln16-Pro288 is expressed with a 6His tag at the C-terminus.
Accession #:P12314
Known as:High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; Fc-gamma RI; FcRI; Fc-gamma RIA; FcgammaRIa; CD64; FCGR1A
Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Quality Control
Purity:Greater than 95% as determined by reducing SDS-PAGE.
BioActivity:Immobilized Human SIgG1-Fc (Cat#CX82) at 2μg/ml (100 μl/well) can bind Human CD64-His(Cat#CM03).
Endotoxin:Less than 0.1 ng/?g (1 EU/?g)
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background
CD64 (FcγRI), one of the Fc receptors for IgG, is a membrane glycoprotein that mediates endocytosis, phagocytosis, antibody-dependent cellular cytotoxicity, cytokine release, and superoxide production. CD64 is also structurally distinct, containing an extracellular Ig-interactive region of three Ig-like domains in contrast to the two domains of the low affinity receptors FcγRII and FcγRIII. It is normally expressed on the surfaces of monocytes and macrophages.

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