Recombinant Human CD3 epsilon/CD3E (C-mFc)

Cat.No.: C00E

Recombinant Human CD3E (C-mFc)
Description
Recombinant Human T-Cell Surface Glycoprotein CD3 epsilon Chain is produced by our Mammalian expression system and the target gene encoding Gln22-Thr48 is expressed with a mFc tag at the C-terminus.
Accession #:P07766
Known as:T-Cell Surface Glycoprotein CD3 Epsilon Chain; T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e; CD3E; T3E
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.
Quality Control
Purity:Greater than 95% as determined by reducing SDS-PAGE.
BioActivity:Immobilized Human CD3E-mFc(Cat#C00E) at 10μg/ml (100 μl/well) can bind Human Anti-CD3(Cat#GMP-A018).
Endotoxin:Less than 0.1 ng/?g (1 E
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background
T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

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