Recombinant Mouse ICOS Ligand/B7-H2/CD275 (C-6His)

Cat.No.: CS08

Recombinant Mouse B7-H2 (C-6His)
Description
Recombinant Mouse ICOS Ligand is produced by our Mammalian expression system and the target gene encoding Glu47-Lys279 is expressed with a 6His tag at the C-terminus.
Accession #:Q9JHJ8
Known as:B7 homolog 2; B7-H2;B7-like protein Gl50; B7RP-1LICOS; CD275; CD275 antigen; ICOS ligand; ICOSL; ICOS-L;inducible T-cell co-stimulator ligand; B7RP-1; CD275; GL50; ICOSL; ICOSLG; B7H2;B7RP1
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Quality Control
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background
Mouse ICOS ligand(B7-H2) is an approximately transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, monocytes, and dendritic cells. It binds to ICOS on activated T cells, leading to both positive and negative effects on immune responses including its own down-regulation. The B7-H2 interaction with ICOS is costimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells and germinal centers. B7-H2 contributes to the development of allergic asthma by enhancing Th2 biased immune responses, limiting Th17 responses, and promoting eosinophilic infiltration into the lung. Its activation of ICOS on Treg limits pulmonary inflammation and airway hyperresponsiveness, promotes the development of inhalational tolerance, and impairs antitumor immunity. In the thyroid, B7-H2 is up-regulated on thyrocytes during inflammation and promotes their proliferation and production of thryoid hormones.

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