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Pyrophosphatase, Inorganic (yeast), GMP Grade(Cat. No.:GMP-M036)
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Description
  • Inorganic Pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates: P2O74-+H2O+PPase→2HPO42-. Applied in nucleic acid amplification experiments, PPase can hydrolyze the inorganic pyrophosphate generated by the polymerization to avoid its inhibition effect to synthesis of RNA strand.
  • Pyrophosphatase, Inorganic (yeast) is GMP Grade produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.
  • This product has completed the DMF record of FDA and passed the HALAL certification.
Quality Control
ElementStandard
AppearanceClear and transparent solution
IdentificationPositive
Visible ParticlesMeet the specification
pH7.5-8.5
Activity98U/ml-102U/ml
Purity≥95%
Protein ContentMeet the specification
Endonuclease ResiduesThe degradation of substrate was ≤10%
Exonuclease ResiduesThe degradation of substrate was ≤10%
RNase ResiduesThe degradation of substrate was ≤10%
Bacterial Endotoxins<5EU/ml
Exogenous DNA Residues≤100pg/mg
Host-cell Protein Residues≤50ppm
MycoplasmaNegative
Heavy Metals≤10ppm
Microbial LimitTotal aerobic microbial count≤1cfu/10ml, total yeasts and molds count≤1cfu/10ml
Storage
At -20±5℃.
Components
SKUComponentsVolume
GMP-M036-01APyrophosphatase, Inorganic (yeast), GMP Grade (0.1U/μl)100μl
GMP- M036-M001Pyrophosphatase, Inorganic (yeast), GMP Grade (0.1U/μl)1ml
GMP- M036-M010Pyrophosphatase, Inorganic (yeast), GMP Grade (0.1U/μl)10ml
GMP- M036-M050Pyrophosphatase, Inorganic (yeast), GMP Grade (0.1U/μl)50ml
Notes
  • The enzyme shows bioactivity in various reaction buffers. Usually, the enzyme can be directly added in HDA amplification, LAMP amplification, etc.
  • The amount of the enzyme needs to be optimized in different reactions, possibly functional at a concentration of 0.05 ~ 1U/ml.
  • The optimal reaction temperature of this enzyme is 25℃, it is active at 16 ~ 37℃, and the enzyme can be inactivated by 65℃ for 10min.
  • Mg2+ is indispensable for bioactivity of this enzyme.
Related Products
Cat. No.Product NameCat. No.Product Name
GMP-M062Vaccinia Capping Enzyme, GMP GradeGMP-E125RNase Inhibitor, GMP Grade
GMP-M072mRNA Cap 2´-O-Methyltransferase, GMP Grade
GMP-E127DNase I, GMP Grade
GMP-M012E. coli Poly(A) Polymerase, GMP GradeGMP-E121T7 RNA Polymerase, GMP Grade
GMP-S023-026NTPs, GMP Grade
仅供科研或生产使用,不可直接应用于人体。
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