Recombinant Human Carbonic Anhydrase IX/CA9 (C-6His)

Cat.No.: C02X

Recombinant Human Carbonic Anhydrase 9 (C-6His)
Description
Recombinant Human Carbonic Anhydrase 9 is produced by our Mammalian expression system and the target gene encoding Gln38-Asp414 is expressed with a 6His tag at the C-terminus.
Accession #:Q16790
Known as:CA9; CA-IX; Carbonic Anhydrase IX; Carbonate dehydratase IX; G250; MN; P54/58N; RCC; RCC-associated protein G250
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.
Quality Control
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:Less than 0.1 ng/?g (1 EU/?g) as determined by LAL test.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background
Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA9 is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC. Appears to be a novel specific biomarker for a cervical neoplasia.

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