Recombinant 2019-nCoV 2019-nCoV Guanine-N7_meth

Cat.No.: CR83

Description
Recombinant 2019-nCoV Guanine-N7 methyltransferase is produced by our E.coli expression system and the target gene encoding Ala1-Gln527 is expressed with a 6His tag at the N-terminus.
Accession #:YP_009725309.1
Known as:SARS-CoV 2 nsp14; SARS-CoV 2 ExoN; Guanine-N7 methyltransferase
Formulation
Supplied as a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4.
Quality Control
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Storage
Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.
Background
The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.

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