Recombinant 2019-nCoV 2019-nCoV Helicase (C-6His)

Cat.No.: CR84

Description
Recombinant 2019-nCoV Helicase is produced by our E.coli expression system and the target gene encoding Ala1-Gln601 is expressed with a 6His tag at the C-terminus.
Accession #:YP_009725308.1
Known as:SARS-CoV 2 Helicase; SARS-CoV 2 nsp13
Formulation
Supplied as a 0.2 μm filtered solution of .
Quality Control
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Storage
Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.
Background
The non—structural protein 13 (nsp13) of SARS—CoV 2 is a helicase that separates double—stranded RNA or DNA with a 5'—3' polarity, using the energy of nucleotide hydrolysis. A basic biochemical characterization of nsp13 demonstrated that it can unwind both doublestranded DNA and RNA in a 5’-3’ direction, and it can hydrolyze all deoxyribonucleotide and ribonucleotide triphosphates. Helicases are motor proteins that utilize the energy derived from nucleotide hydrolysisto unwind double-stranded nucleic acids into two single-stranded nucleic acids. Initially, helicases were only thought to be molecular engines that unwind nucleic acids during replication, recombination, and DNA repair. Recent studies have shown that they are also involved in other biological processes, including displacement of proteins from nucleic acid, movement of Holliday junctions, chromatin remodeling, catalysis of nucleic acid conformational changes, several aspects of RNA metabolism, including transcription, mRNA splicing, mRNA export, translation, RNA stability and mitochondrial gene expression. Some human diseases, including Bloom’s syndrome, Werner’s syndrome, and Xeroderma Pigmentosum have been associated with defects in helicase function.

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